Rapid and simultaneous determination of tetra cyclic peptide phytotoxins, tentoxin, isotentoxin and dihydrotentoxin, from Alternaria porri by LC/MS

نویسندگان

  • Masayuki Horiuchi
  • Naoshige Akimoto
  • Keiichiro Ohnishi
  • Masakazu Yamashita
  • Takashi Maoka
چکیده

In the course of our investigation on bioactive products of Alternaria porri , the causal fungus of black spot disease in the stone− leek and onion, we have reported the isolation of cyclic tetra peptide, tentoxin [cyclo−(L−leucyl−N −methyl−(Z )−dehydrophenylalanyl−glycyl−N −methyl−L−alanyl), 1] [1]. Subsequently, we isolated and identified two tentoxin related cyclic peptide, isotentoxin [cyclo−(L−leucyl−N −methyl−(E )−dehydrophenylalanyl−glycyl−N Original

برای دانلود متن کامل این مقاله و بیش از 32 میلیون مقاله دیگر ابتدا ثبت نام کنید

ثبت نام

اگر عضو سایت هستید لطفا وارد حساب کاربری خود شوید

منابع مشابه

Development and Validation of a LC-ESI-MS/MS Method for the Determination of Alternaria Toxins Alternariol, Alternariol Methyl-Ether and Tentoxin in Tomato and Tomato-Based Products

Alternaria species are capable of producing several secondary toxic metabolites in infected plants and in agricultural commodities, which play important roles in food safety. Alternaria alternata turn out to be the most frequent fungal species invading tomatoes. Alternariol (AOH), alternariol monomethyl ether (AME), and tentoxin (TEN) are some of the main Alternaria mycotoxins that can be found...

متن کامل

Putative Nonribosomal Peptide Synthetase and Cytochrome P450 Genes Responsible for Tentoxin Biosynthesis in Alternaria alternata ZJ33

Tentoxin, a cyclic tetrapeptide produced by several Alternaria species, inhibits the F₁-ATPase activity of chloroplasts, resulting in chlorosis in sensitive plants. In this study, we report two clustered genes, encoding a putative non-ribosome peptide synthetase (NRPS) TES and a cytochrome P450 protein TES1, that are required for tentoxin biosynthesis in Alternaria alternata strain ZJ33, which ...

متن کامل

Method development for simultaneous determination of 41 pesticides in rice using LC-MS/MS technique and its application for the analysis of 60 rice samples collected from Tehran market

For the first time, a multi-residue methodfor simultaneous determination of 41LC-amenable pesticides in rice, belonging to different chemical classes has been developed in Iran by LC-MS/MS. The pesticides were analyzed simultaneously in a single run using positive electrospray ionization with multiple reaction monitoring (MRM) after extraction with slightly modified QuEChERS method. The calibra...

متن کامل

Method development for simultaneous determination of 41 pesticides in rice using LC-MS/MS technique and its application for the analysis of 60 rice samples collected from Tehran market

For the first time, a multi-residue methodfor simultaneous determination of 41LC-amenable pesticides in rice, belonging to different chemical classes has been developed in Iran by LC-MS/MS. The pesticides were analyzed simultaneously in a single run using positive electrospray ionization with multiple reaction monitoring (MRM) after extraction with slightly modified QuEChERS method. The calibra...

متن کامل

Structure of spinach chloroplast F1-ATPase complexed with the phytopathogenic inhibitor tentoxin.

Tentoxin, a natural cyclic tetrapeptide produced by phytopathogenic fungi from the Alternaria species affects the catalytic function of the chloroplast F(1)-ATPase in certain sensitive species of plants. In this study, we show that the uncompetitive inhibitor tentoxin binds to the alphabeta-interface of the chloroplast F(1)-ATPase in a cleft localized at betaAsp-83. Most of the binding site is ...

متن کامل

ذخیره در منابع من


  با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید

برای دانلود متن کامل این مقاله و بیش از 32 میلیون مقاله دیگر ابتدا ثبت نام کنید

ثبت نام

اگر عضو سایت هستید لطفا وارد حساب کاربری خود شوید

عنوان ژورنال:

دوره   شماره 

صفحات  -

تاریخ انتشار 2004